Fig. 2.

Effects of L-Cysteine administration on neuronal cell loss and ultrastructural changes in neurons of the ipsilateral cortex. (A) Representative Nissl staining of brain sections performed 3 days after HI insult. Scale bar = 2000 µm. (B) RepresentativeTEM results from each group showing the nucleus (N), rough endoplasmic reticulum (ER), mitochondria (M), and ribosomes, n=3 in each group. Arrow indicates the necrotic cells, Asterisk (*) indicates swelling and vacuolized mitochondria. Scale bar = 2 µm. (C) The quantification of tissue loss was expressed as (contralateral area − ipsilateral area/contralateral area) × 100. Values representthe mean ± SD. n = 6 in each group. Values represent the mean ± SD, ***p < 0.001 HI VS Sham; ##p < 0.01, ###p < 0.001 HI + L-Cys VS HI; &&&p < 0.001 HI + L-Cys+AOAA VS HI + L-Cys.