Fig. 3.

Effects of L-Cysteine administration on CBS expression and H₂S production after HI. The CBS levels of the ipsilateral cortex were measured 3 days after HI by Western blot (A), RT-PCR (B) and immunohistochemical staining (C). Bar graphs show quantification of CBS levels from Western blot and RT-PCR. n = 4 in each group. Scale bar = 50 µm. (D) Quantitative analysis of the number of CBS-positive cells. Values are expressed relative to those of the Sham group. n = 4 in each group. (E) The CBS activity of the ipsilateral cortex was measured by a commercial kit, n = 6 in each group. (F) The content of H₂S in the ipsilateral cortex was measured by the NNDPD method, n = 6 in each group. Values represent the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001 HI VS Sham; #p < 0.05, ##p < 0.01, ###p < 0.001 HI + L-Cys VS HI; &p < 0.05, &&p < 0.01, &&&p < 0.001 HI + L-Cys+AOAA VS HI + L-Cys.