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. 2017 Jul 20;13:1744806917719847. doi: 10.1177/1744806917719847

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© The Author(s) 2017

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 5. — Repetitive electrical stimulation evoked Ca²⁺ signals in cell population after bolus loading of Cal-520 AM in ACC pyramidal neurons. (a) Schematic diagram showing the placement of stimulating electrode and puffing pipette in the layer II/III pyramidal neurons of ACC. (b) Left: two-photon fluorescent image of puff-application of calcium indicator Cal-520 AM (200 µM). The blue dash circle indicates the position of the frame scan: Right, waveforms of fluorescence changes (ΔF/F) in response to five traces at 5, 10, 20, and 50 Hz in single cell. (c) Left: representative fluorescent image of cell population showing the soma of active neurons. Right: waveforms of fluorescence changes (ΔF/F) in response to five traces at 50 Hz in four active cells. (d) Pie graph summarizing the active ratio of evoked Ca²⁺ signals in cell population (n = 42) after five traces at 50 Hz electrical stimulation.