Fig 6. EBNA3C promotes cellular proliferation by upregulating Bcl6-targeted Bcl2 expression.
A) Saos-2 cells were transfected with the indicated plasmids in combination with the GFP vector and selected with G418 antibiotic for 2 weeks. The GFP fluorescence of each plate was scanned by PhosphorImager and the colonies were calculated by Image J software. B) 105 selected cells from the former experiment were plated and cultured for 10 days. Viable cells were counted at the indicated time using trypan blue staining. C) HEK293 cells were transfected, selected and counted the numbers. D) 10 million LCL1 cells were treated with 50 μg/ml (110 μM) Bcl6 inhibitor for 15 hours. Then cells were harvested, and western blot analysis was performed with indicated antibodies. These results shown are representative of three independent experiments.