Fig 1. Identification of multiple transcripts originating for the Dkk3 gene locus.

(A) Schematic diagram of the Dkk3 gene (NC_000073.6) in the wild type and Dkk3^tm1Cni mouse. Initiator methionine’s for Dkk3 (NM_0154814) and D2p29 (AF245040) indicated by arrows; CpG islands indicated by red box; LacZ-pA stop cassette in yellow. (B) Quantitative PCR of Dkk3 containing exon 2 and exon 3 transcripts in total brain RNA from the Dkk3^tm1Cni mouse. Arrows indicate PCR primer sites. Data reported as means ± se of 3 individuals; each sample determined in quadruplicate. (C) Schematic diagram of rat Dkk3 intron 2-luciferase reporter constructs. Arrows indicate orientation and location of intron 2 segments upstream of exon 3; data reported as means ± se of 3 independent experiments, each sample determined in triplicate. (D) ChIP analysis of RNA pol II and TATA box binding protein (TBP) binding to the ~66 nucleotides (nt6682-nt6948) of intron 2 adjacent to exon 3 in the rat astrocyte Dkk3 gene; data reported as means ± se of 3 independent experiments; each sample determined in quadruplicate.