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. 2017 Jul 24;12(7):e0181724. doi: 10.1371/journal.pone.0181724

Fig 3. Analysis of the biological role the TSS2 driven Dkk3b in the ZFN gene-edited Dkk3bCFP/+ mouse.

Fig 3

(A) Inhibition of DNA methyltransferase activity increases TSS2-driven CFP in Dkk3bCFP/+ cells. (B) DKK3 isoforms present in wild type and Dkk3bCFP/mCherry MEFs (anti-DKK3 (ab186409) (Abcam). (C) QPCR analysis of Dkk3 and Dkk3b transcripts in wild type and Dkk3bCFP/mCherry MEFs. Data reported as means ± se, n = 3. (D) TSS2-driven, immunoreactive CFP expression in representative tissues of the Dkk3bCFP/wt mouse. NSB, Normal rabbit serum, CFP, anti-CFP IgG(cat#632381) (TakaRa).