Fig 6. DKK3b effects on Wnt1-stimulated ß-catenin signaling.

(A) Representative photomicrographs of the cellular distribution of transiently transfected DKK3b and TAT-DKK3b in HEK293 cells. DKK3b identified with anti-Flag IgG and anti-DKK3 IgG, respectively. (B) DKK3b blocks Wnt1-stimulated cell proliferation without altering basal cell proliferation. Data reported as means ± se of 4 independent experiments; n = 3 in individual experiments. Open bar—day 1; shared bars—day 3. (C) TAT-DKK3b antagonizes Wnt1-stimulated TOPflash activity and secondary ß-catenin dependent gene expression. Data reported as means ± se of 3 independent experiments; triplicate determinations done in individual experiments.