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. 2017 Jul 24;12(7):e0181724. doi: 10.1371/journal.pone.0181724

Table 1. Primers used in this study.

Primer use sequence
5HR7351F 5 HR arm 5’-AGAGAGAGAGAGACTAAGGTACTGGC
5HR38275R 5 HR arm 5’-GATCCTGAACCGGTATAACTTCGTATAATGTATGCTATACGAAG-TTATCAGTTACAACTGAGGTGAAGAAAAGG
3HR8276LAF 3 HR arm 5’-CCTGACTTAAGATAACTTCGTATAGCATACATTATACGAAGTTA-TAAAGAAACCGTTTTGTGTCTTTGATTTG
3HR9050 3 HR arm 5’-GATCCTGACTTAAGTCAAGTCATTCAGGCTGGTTG
LF Dkk3 locus 5’-AAGGACCCTGCCTTGGCCACTTGG
LR Dkk3 locus 5’- ACGGCATGGACGAGCTGTACC
RF Dkk3 locus 5’- GCTGTACCGCTAAAGCGGCCGC
RR Dkk3 locus 5’- CTCCACCCAGCTCCTGATTC
DPF1 genotyping 5’-TTTGGCTTGCTGGCTAAGAT
DPR2 genotyping 5’-GAGGGTGGTCACCAGGGT
DCF3 genotyping 5’-CGCCACAACATCGAGG
DCF4 genotyping 5’-ACCATGAGGTCTCGTCAACC
Dkk3F Off-target PCR 5'-TGTGCCACGGCCCTTACCTT
Dkk3R Off-target PCR 5'-GGCTAAGATAACCCTTCTGAGGTC
DlstF Off-target PCR 5’GCAGAGGAAGTGCAAGTTTAAGGCTAGATC
DlstR Off-target PCR 5’CAGCCATTCTAGAAACCCTGATCAAG
Rnu6F Off-target PCR 5'-AAACTGGCTCTCGTGGGGGT
Rnu6R Off-target PCR 5'-CACAGGTGACAGTGTGGTGG
Tcf7l2F Off-target PCR 5'-ATGTCAGCCCACAGTGATGAGAG
Tcf7l2R Off-target PCR 5'-CTCCCTGGAAATTGGCAGCTTG
Akap9F Off-target PCR 5'-CCTACAGATCCGTTAAGGGTACAG
Akap9R Off-target PCR 5'-CTCAGAGGAGGAGTCATCAAGTG
Drb1F Off-target PCR 5’-CTCACTGTTTCTAGGAACCTTGGTTCTGTC
Drb1R Off-target PCR 5’-GAACAGAGAAAGAAAGTCTGAGCTCAGTCC
Aldoart1F Off-target PCR 5’-GCAGTGGCTATAGCAGAGAGGAAGAA
Aldoart1R Off-target PCR 5’-TGCCGATGTCAAATGTAGCATGGCACTATC
Fam168bF Off-target PCR 5'-GCATCTGTCTGCAGACCACATTTG
Fam168bR Off-target PCR 5'-GCACGTTCACAATACTGTGGGG
Mett21eF Off-target PCR 5’GAGAGAGAGAGAGAGAGAGAGAGAGAAGAA
Mett21eR Off-target PCR 5’CAGAGTTAGCATTTCTTCTAGGCTCCACC
Rps6ka5F Off-target PCR 5'-GAGGCGCATGCAGAAACTTCAC
Rps6ka5R Off-target PCR 5'-CCTGAAAAGCCTACTAGGTCTCTC
Arhgef2F Off-target PCR 5'-AGAGAGAGAGAGAGAGAGAGAGAG
Arhgef2R Off-target PCR 5'-GGTGGCAAGAACCAGATGTGCT
TSS2F CHiP 5’-AGATGGCCCCTTTTCTTCAC
TSS2R CHiP 5’-TGGTCTCGTTGTGGTAGTTGG
EXF2 qPCR 5’-CCAGCTCTCAACTACCCTCA
EXR2 qPCR 5’-CATCAGCTCCTCCACCTCTCG
EXF3 qPCR 5’-GCTGCTAAAACGTCCTCTGAGG
EXR3 qPCR 5’-GTCTCCGTGCTGGTCTCATT
RActF qPCR 5’-CTAAGGCCAACCGTGAAAAG
RActR qPCR 5’-GGGGTGTTGAAGGTCTCAAA
GAPDHF qPCR 5’-TGCCACTCAGAAGACTGTGG
GAPDHR qPCR 5’-GGATGCAGGGATGATGTTCT
DKSF loxP scar 5'-GAGAAGGCAGCCCCTTTTCT
DKSR loxP scar 5'-CTTCTTCCGCCTCCATCTATCAAATC