Maximum likelihood phylogenetic tree of 16S rRNA gene sequences obtained from eleven single amplified genomes (orange), one MDA screen (DSC9), draft genomes recently described from amended subterranean aquifers as part of the Parcubacteria superphylum (rRNA genes >1000bp, Brown et al., 2015) and previously described single cell and composite genomes (green; Rinke et al., 2013, Wrighton et al., 2012). The OD1-DSC clade falls into a separated unnamed/unclassfied lineage, a division supported by additional phylogenies (figures S3–S5). Scale bar represents 0.04 changes per position. Confidence values above 50% are shown at the tree nodes. The tree was rooted using Escherichia coli K12.