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First round PCR (A) and semi-nested PCR (B) amplified products using Theileria lestoquardi specific primers. Lane M- 50bp DNA ladder (Fermentas, Germany), A) Lane 1: Positive control, Lane 2: Infected goat blood, Lane 3: Negative control, Lane 4: Infected H. marginatum, Lane 5: Infected R. turanicus, Lane 6: Infected D. marginatus. B) Lane P: Positive control, Lane N: Negative control, Lanes 1, 2: Infected goats blood, Lanes 3, 4: Infected ticks
