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. Author manuscript; available in PMC: 2017 Sep 12.
Published in final edited form as: Angew Chem Int Ed Engl. 2016 Aug 5;55(38):11387–11391. doi: 10.1002/anie.201604014

Table 1.

Michaelis-Menten parameters for the VFD vs. non-VFD mediated processing of β-glucosidase.*

Parameter Non VFD-mediated reaction VFD-mediated rate acceleration
Vmax (nM s−1) 128 ± 5.71 309 ± 52.4
Km (mM) 3.76 ± 0.15 2.50 ± 0.44
kcat (s−1) 13.4 ± 0.59 32.1 ± 5.45
kcat/Km (mM−1s−1) 3.55 ± 0.19 13.32 ± 4.03
*

For this VFD-process, β-glucosidase (9.26 nM), a rotational speed of 7.60 krpm was used, as this provided the most consistent enhancement over sustained time periods. Error indicates the standard deviation around the mean (n=3). There was no overlapping error at 95% confidence limits. The raw data was fitted to a Michaelis-Menten plot and analyzed using a least squares fitting (LSF) approach (Figures S17 and S17).