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. 2017 Jul 24;7:6301. doi: 10.1038/s41598-017-06443-1

Figure 2.

Figure 2

L. pneumophila induced cytokines elicit a pro-inflammatory response in non-infected A549 cells. (a,b) Response of A549 cells to vesicle-free supernatant or 100 k pellet from L. pneumophila-infected THP-1 cells. A549 cells were stimulated with vesicle-free supernatant or 100 k pellet from THP-1 cells infected with L. pneumophila (L.p.) at an MOI of 0.25 and 0.5, respectively, for 24 h. A549 cells were incubated as indicated for 24 or 48 h, respectively. RNA and supernatant were collected. (a) qPCR was performed for expression of CXCL8, miR-146a and pri-mir-146a. (b) Magnetic multiplex ELISA was performed. Results for IL-6, GM-CSF and MCP-1 are shown. Data are shown as mean + SEM of at least three independent experiments. *p < 0.05, **p < 0.01. *Compared to corresponding control, #compared to corresponding supernatant treated sample.