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. 2017 Jul 24;7:6301. doi: 10.1038/s41598-017-06443-1

Figure 3.

Figure 3

THP-1-derived IL-1β induces pro-inflammatory response in A549 cells. (a,b) Response of A549 cells to vesicle-free supernatant from L. pneumophila-infected THP-1 cells. THP-1 cells were infected with L. pneumophila (MOI 0.5) for 24 h. A549 cells were pre-incubated for 1 h with a neutralizing TNF-α antibody (α-TNF-α) or an IL-1 receptor antagonist (IL-1RA) alone or in combination, respectively. Cells were then incubated with the vesicle-free supernatant for 24 or 48 h, respectively. RNA was collected and used for expression analysis of CXCL8 (a) and miR-146a (b). Data are shown as mean + SEM of three independent experiments. ***p < 0.001, ****p < 0.0001 compared to corresponding supernatant treated cells.