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. 2017 Jul 24;7:6301. doi: 10.1038/s41598-017-06443-1

Figure 5.

Figure 5

Block of exosome release does not reduce the THP-1 response to the 100 k pellet. (a) THP-1 cells were incubated with GW4869 (10 or 15 µM) or DMSO as a solvent control for 1 h before infection with L. pneumophila (MOI 0.5) for 24 h. A 100 k pellet was generated and Alix expression was analysed by western blot. Densitometric quantification of western blot bands is shown. Exosome secretion is reduced after GW4869 treatment. (b,c) Donor THP-1 cells were pre-incubated with GW4869 (15 µM) or the solvent control for 1 h before infection with L. pneumophila for 24 h. EVs were purified by differential centrifugation and added to THP-1 (b) or A549 (c) cells. CXCL8 expression was analysed by qPCR after 24 or 48 h of incubation. Data are shown as mean + SEM of three independent experiments. *p < 0.05, **p < 0.01, ns: not significant.