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. 2017 Jul 24;7:6197. doi: 10.1038/s41598-017-06262-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Functionality and subcellular distribution of GLUT5 variants. (a) Suspensions of EBY.VW4000 cells (OD_600nm = 1) transformed with plasmids encoding GLUT5tr, GLUT5tr^S72Y, GLUT5tr^S76I or their fusions with sGFP were droped onto agar plates containing 2% (w/v) fructose (SCF-Ura-Met medium) or 1% (w/v) maltose (SCM-Ura medium). The latter medium was used to control the viability of the transformants and empty vector (V) was a negative control for growth on fructose. The duration of incubation at 30 °C is indicated. (b) The cells expressing the indicated variant of GLUT5-sGFP fusion proteins were cultivated in SCM-Ura-Met media until the exponential phase and analyzed by confocal laser scanning microscopy. Scale bar, 5 µm.