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. 2017 Jul 24;7:6197. doi: 10.1038/s41598-017-06262-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transport kinetics and inhibition of GLUT5 mutants in hxt ⁰ cells. The initial uptake velocity v_t (expressed as nmol fructose transported per minute per mg cell dry weight) was measured for GLUT5tr^S72Y (a) and GLUT5tr^S76I (b) and plotted against sugar concentration. Mean values and standard deviation were calculated from biological triplicates. The lines represent a least-square fit to the Michaelis-Menten equation. The uptake of fructose was measured at variable MSNBA (c) and ECG (d) concentrations and the uptake velocity was normalized to that in the absence of the inhibitor. The IC₅₀ values were calculated by nonlinear algorithm plots supplied by Prism (GraphPad Software).