Table 2.

DCUK-OEt (0.3 μM) induced change in the response to EC₁₀ GABA in GABA_A receptors composed of different subunit combinations.

Receptor	Percent Change	Standard Error	Sample Size	Unadjusted p-value	Bonferroni adjusted p-value	Effect
α1β2	40	19	11	0.035	0.42	Non-significant
α1β3	20	27	5	0.449	>0.99	Non-significant
α5β3	8	25	8	0.730	>0.99	Non-significant
α1β1γ2	17	23	11	0.445	>0.99	Non-significant
α1β2γ2	127	10	44	<0.001	<0.01	Significant
α1β2(N265S)γ2	45	18	14	0.013	0.16	Marginal
α1β2γ1	56	24	8	0.022	0.26	Non-significant
α1β3γ2	95	29	6	0.002	0.02	Significant
α4β3γ2	49	26	8	0.066	0.79	Non-significant
α5β3γ2	81	24	8	0.001	0.01	Significant
α1β3δ	102	16	18	<0.001	<0.01	Significant
α4β3δ	−6	31	5	0.842	>0.99	Non-significant

Significant and marginal effects are those with a Bonferroni-adjusted p-value < 0.05 and < 0.2, respectively. A linear mixed model was implemented in SAS (version 9.4) to calculate the normalized percent change in current for each receptor subunit combination produced by DCUK-OEt (EC₁₀ GABA concentration without and with 0.3 μM DCUK-OEt). A random effect of batch was included in the model, and for each receptor, the percent change in the GABA-induced current produced by DCUK-OEt was compared to 0 using a single-sample t-test in the MIXED procedure in SAS and a Bonferroni adjustment to correct for multiple comparisons.