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. 2017 Jul 25;8:523. doi: 10.3389/fphys.2017.00523

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Copyright © 2017 Lejay, Laverny, Paradis, Schlagowski, Charles, Singh, Zoll, Thaveau, Lonsdorfer, Dufour, Favret, Wolff, Metzger, Chakfe and Geny.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Oxidative stress is increased by critical limb ischemia and exercise. Production of free radicals (A) was increased in ischemic muscles (n = 10) compared to control muscles (n = 10) in the non-exercised group. DHE staining (B) showed higher fluorescence in ischemic muscles compared to control muscles (a, Control limb; b, Ischemic limb) in the non-exercised group. Production of free radicals was increased in the exercised group compared to the non-exercised group (A). Exercice showed higher fluorescence (B) in control and ischemic limbs (c, Control limb + exercise; d, Ischemic limb + exercise). ^*p < 0.05; ^**p < 0.01.