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. 2017 Jul 25;8:1353. doi: 10.3389/fmicb.2017.01353

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Copyright © 2017 Mangalea, Plumley and Borlee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Complementation of the I1013::T24 (narL) and I1014::T24 (narX) mutants that are resistant to the inhibitory effects of exogenous nitrate. Mutants of the predicted nitrate-sensing two-component system narX/narL were complemented with full-length Bp1026b_I1013 (narL) and Bp1026b_I1014 (narX). The genes were re-introduced in the T24-mutant background at a neutral site and expression was conditionally induced using 5 mM IPTG. Biofilm formation was assessed in the presence and absence of 5 mM NaNO₃. Asterisks indicate a significant difference (p < 0.0001) calculated with an unpaired Student’s t-test.