Table 1.
Predicted nitrate metabolism genes identified in this study.
| Name and predicted function | Putative annotationa | B. pseudomalleib | P. aeruginosa | Nucleotide similarityc(%) |
|---|---|---|---|---|
| DNA-binding response regulator | narL | Bp1026b_I1013 | PA3879 | 65.12 |
| Nitrate/nitrite sensor protein | narX | Bp1026b_I1014 | PA3878 | 65.00 |
| Nitrate reductase (1) | ||||
| Gamma subunit | narI-1 | Bp1026b_I1015 | PA3872 | 64.50 |
| Delta subunit | narJ-1 | Bp1026b_I1016 | PA3873 | 72.54 |
| Beta subunit | narH-1 | Bp1026b_I1017 | PA3874 | 75.88 |
| Alpha subunit | narG-1 | Bp1026b_I1018 | PA3875 | 75.20 |
| Nitrate/nitrite transporter | narK-2d | Bp1026b_I1019 | PA3876 | 73.90 |
| Nitrate/nitrite transporter | narK-1d | Bp1026b_I1020 | PA3877 | 69.76 |
| Nitric oxide reductase | norB | Bp1026b_I0974 | PA0524 | 68.37 |
| Nitrous oxide reductase | nosZ | Bp1026b_I1546 | PA3392 | 68.47 |
| Assimilatory nitrite reductase (NAD(P)H) (1) | ||||
| Large subunit | nirB-1 | Bp1026b_I2984 | PA1781 | 76.39 |
| Small subunit | nirD-1 | Bp1026b_I2985 | PA1780 | 69.23 |
| Assimilatory nitrate reductase (1) | ||||
| Molybdopterin oxidoreductase family protein | nasA-1e | Bp1026b_I2986 | PA1779 | 67.45 |
| Nitrate/nitrite transporter | narK | Bp1026b_II1220 | PA3870 | 59.89 |
| Nitrate reductase (2) | ||||
| Gamma subunit | narI-2 | Bp1026b_II1222 | PA3872 | 65.45 |
| Delta subunit | narJ-2 | Bp1026b_II1223 | PA3873 | 65.60 |
| Beta subunit | narH-2 | Bp1026b_II1224 | PA3874 | 78.55 |
| Alpha subunit | narG-2 | Bp1026b_II1225 | PA3875 | 74.14 |
| Assimilatory nitrate reductase (2) | ||||
| Large subunit | nasA-2 | Bp1026b_II1316 | PA1779 | 66.77 |
| Assimilatory nitrite reductase (NAD(P)H) (2) | ||||
| Small subunit | nirD-2 | Bp1026b_II1317 | PA1780 | 64.53 |
| Large subunit | nirB-2 | Bp1026b_II1318 | PA1781 | 62.74 |
| Nitrate regulator | nasTd | Bp1026b_II1319 | PA1783 | 61.92 |
| Nitrate transport ATP-binding protein | nasS | Bp1026b_II1322 | PA1786 | 68.54 |
| Nitrite reductases | ||||
| Multicopper oxidase domain-containing protein | nirSd | Bp1026b_II1540 | PA0519 | 60.60 |
| Anaerobically induced outer membrane protein | nirKf | Bp1026b_II1580 | N/A | N/A |
aGene annotations determined using the Burkholderia Genome Database.
bLocus ID determined using GenBank Database (NCBI – http://www.ncbi.nlm.nih.gov/) in conjunction with the Burkholderia Genome Database.
cPercent identity determined using MUSCLE (Multiple Sequence Comparison by Log-Expectation – http://www.ebi.ac.uk/).
dNo clear annotations in Burkholderia spp.; Annotation from the Pseudomonas Genome Database.
eBP1026_I2986 and its homolog BPSL0510 (see Supplementary Material) are predicted orthologs of Pseudomonas aeruginosa assimilatory nitrate reductase NasA [see Andreae et al., 2014].
fGene specific to Burkholderia spp.