Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 24;7:6294. doi: 10.1038/s41598-017-06546-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Functional analysis of hepatocyte-like cells (HLCs) derived from E-iPSCs. (A) E-Cadherin (E-CAD) antibody staining marking cell shape (left panel), Glycogen storage (right panel), Periodic Acid-Schiff (PAS) assay was used. Glycogen storage is indicated by pink or dark red-purple cytoplasm. (B) Visualization of 5 (and 6)-Carboxy-2′,7′-dichlorofluorescein diacetate (CDFDA), immunofluorescence image of HLCs direct after incubation with CDFDA (left panel), and immunofluorescence image of HLCs six hours later (right panel). (C) Immunofluorescence-based protein staining of bile salt export pump (BSEP). (D) Analysis of UREA production in E-iPSC-DE (DE), E-iPSC-HE (HE) and E-iPSC-HLCs (HLC). Three biological replicates in technical triplicates of each sample were analyzed. The levels of urea are presented as a percentage, considering measured levels of urea in mg/dL/24 h. The error bars indicate the standard errors of the mean. (E) Measurement of bile acid secretion of E-iPSC-HLCs (HLC). (F) Measurement of CYP3A4 secretion of HLC samples. Three biological replicates in technical triplicates were analyzed. The levels of CYP3A4 are presented as relative light units per milliliter (R.L.U./ml). The error bars indicate the standard errors of the mean. (G) Quantitative real-time PCR (qPCR) analysis of cytochrome P450 family member activity of all stages E-iPSC-DE (DE), E-iPSC-HE (HE) and E-iPSC-HLC (HLC) are shown. Three biological replicates in technical triplicates of each sample were analyzed. The data were normalized to E-iPSCs. The standard deviation is depicted by the error bars.