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. 2017 Jul 25;8:1320. doi: 10.3389/fpls.2017.01320

FIGURE 2.

FIGURE 2

High temperature activates the expression of LNG1 and LNG2 in a PIF4 dependent manner. (A) Diagram showing plant growth conditions used for the qRT-PCR analyses in (B) and (F). Seedlings were maintained under 12 h light/12 h dark conditions at 20°C for 4 days, and then transferred to 24 h light conditions. The seedlings were then exposed for 1 or 4 h to high temperature (28°C) from ZT20 and harvested for RNA extraction. (B–D) The qRT-PCR analysis of the expression levels of PIF4, LNG1, and LNG2 in WT seedlings under the indicated growth conditions. Gene expression levels were normalized to PP2A and presented as values relative to those of the WT seedlings at 20°C at ZT20. Error bars indicate standard deviation (SD, n = 3). (E,F) The qRT-PCR analysis of the expression levels of LNG1 and LNG2. WT and pif4 mutant seedlings were exposed for 4 h to high temperature (28°C) from ZT20 or kept at 20°C. Error bars indicate SD (n = 3). Different letters above each bar indicate statistically significant differences (ANOVA and Tukey’s HSD; P < 0.05).