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. 2017 Jul 25;8:1320. doi: 10.3389/fpls.2017.01320

FIGURE 3.

FIGURE 3

LONGIFOLIA activities are required for thermomorphogenic growth. (A) Diagram showing plant growth conditions for hypocotyl length measurements. WT and lngq seedlings were grown at 20°C for 7 days or grown at 20°C for 4 days followed by incubation at 28°C for 3 days before harvesting for hypocotyl length measurements. (B) Representative seedlings grown under the conditions described in (A). (C) Average hypocotyl lengths of WT and lngq seedlings grown under the conditions described in (A). Error bars indicate standard deviation (SD, n = 10 plants). Numbers indicate ratios of hypocotyl lengths (28°C/20°C). Different letters above each bar indicate statistically significant differences (ANOVA and Tukey’s HSD; P < 0.05). (D) Representative leaf blades and petioles of wild type and lngq plants grown at 20°C or 28°C for 2 weeks. Bar indicates 5 mm. (E) Average petiole lengths of WT and lngq plants grown at two different temperatures. Error bars indicate SD (n = 20 petioles). Numbers indicate ratios of petiole lengths (28°C/20°C). Different letters above each bar indicate statistically significant differences (ANOVA and Tukey’s HSD; P < 0.05). (F) Average ratio of the leaf length to width of plants grown at 20°C or 28°C. The third and fourth leaves were analyzed. Error bars indicate SD (n = 10 leaves). ∗P < 0.05 (Student’s t-test) and ns indicates not significant.