Table 1.
Predicted ZIKV NS1 epitopes from consensus ZIKV NS1 sequence, Dar et al., and Dikhit et al.
| NS1 Residues | Sequence | HLA Class | Flaviviridae Homology (E-value <1)? | World Coverage | South America Coverage | Immunogenicity |
|---|---|---|---|---|---|---|
| 16–24/25 | KETRCGTGV/KETRCGTGVF* | I | DENV2-4 NS1 | 20.88% | 10.34% | 0.09664/0.13444 |
| 125–133 | KSYFVRAAK* | I | None | 38.48% | 28.66% | 0.2614 |
| 158–167 | FLVEDHGFGV**@ | I | DENV1-4 NS1 | 41.35% | 21.85% | 0.31775 |
| 166–175 | GVFHTSVWLK* | I & II | DENV1&3-4 Peptidase S7, NHUV glycoprot. | 69.67% | 78.11% | 0.16657 |
| 169–177 | HTSVWLKVR** | I & II | DENV1-3 NS1 | 58.80% | 79.86% | 0.03522 |
| 1059 | KGPWHSEEL | I | None | Dar et al.26 Polyprotein position used in residue numbering All peptides bind >3 alleles | ||
| 878 | VQLTVVVGS | II | None | |||
| 878 | VQLTVVVGSVKNPM | II | None | |||
| 965 | VREDYSLEC | II | None | |||
| 982 | VKGKEAVHS | II | None | |||
| 1004 | WRLKRAHLI | II | None | |||
| 1045 | LSHHNTREG | II | None | |||
| 1124 | WYGMEIRPR | II | WNV NS1 | |||
| 80–88 | ILEENGVQL | I | None | Dikhit et al.25 NS1 protein position used in residue numbering All peptides bind >3 alleles | ||
| 87–95 | QLTVVVGSV | I | None | |||
| 364–372 | SLGVLVILL | I | None | |||
| 366–374 | GVLVILLMV | I | None | |||
| 370–378 | ILLMVQEGL | I | None | |||
*Exact MHC-NP Matches.
**Near MHC-NP Matches.
@Due to high immunogenicity and DENV1-4 homology, this peptide may serve as a positive control for DENV/ZIKV infection.
Immunogenicity >0 is the threshold for predicted immunogenicity.