Table 2.
Predicted DENV NS2a epitopes for DENV1-4 and consensus DENV NS2a sequence.
| Serotype | Residues | Sequence | MHC II Binding? | ZIKV Homology (E-value <1)? | Other DENV Homology (E-value <1)? | World Coverage | S. America Coverage | Immunogenicity |
|---|---|---|---|---|---|---|---|---|
| 1 | 31–39/40 | MLMTGTLAV/MLMTGTLAVF** | Yes, <3 alleles | None | None | 86.75% | 67.85% | 0.0651/0.09956 |
| 2 | 33–41 | ILLVAVSFV** | Yes | None | None | 72.68% | 57.84% | 0.04368 |
| 63–72 | TMTDDIGMGV* | No | None | None | 41.35% | 21.85% | 0.08962 | |
| 3 | 31–40 | HMIAGVFFTF* | Yes | None | None | 84.20% | 83.18% | 0.38745 |
| 32–40/41 | MIAGVFFTF/MIAGVFFTFV* | Yes | None | None | 94.28% | 89.85% | 0.31868/0.41236 | |
| 4 | 81–90 | KMSPGYVLGV* | Yes | None | None | 51.64% | 30.96% | 0.01384 |
| Consensus | 15–23/24 | MAIFIEEVM/MAIFIEEVMR | Yes | None | 44.73% | 31.63% | 0.49347/0.41459 | |
| 42–50/51 | LLIMGQLTW/LLIMGQLTWR* | Yes, <3 alleles | NS4b | 66.08% | 78.82% | −0.19622/−0.06156 | ||
| 86–94/95 | MFAVGLLLR/MFAVGLLLRK | Yes | None | 66.08% | 78.82% | 0.06096/0.08688 | ||
| 132–140 | MMLKLVTNF* | Yes, <3 alleles | None | 34.12% | 14.89% | −0.16356 | ||
| 143–152 | YQLWTTLLSL | Yes | None | 78.77% | 86.15% | 0.17843 | ||
| 170–178 | MVLAVVSLF** | Yes, <3 alleles | None | 43.07% | 27.68% | −0.03127 |
*Exact MHC-NP match.
**Near MHC-NP match.
Immunogenicity >0 is the threshold for predicted immunogenicity.