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. 2017 Jul 24;7:6211. doi: 10.1038/s41598-017-06089-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vitro Rt-PA delivery in the full occlusion model. Blood clots were treated with a range of Rt-PA alone in a range of concentrations (0, 3, 30 µg/ml). The blood clot volume was assessed every 5 min for 60 min using high frequency ultrasound imaging. 3D reconstructions of clots were made at T0 and T60 (top). I-J: Control; K-L: Rt-PA; M-N: Rt-PAx10. Data expressed as mean ± SEM were calculated from four independent experiments (bottom).