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. 2017 Jul 24;7:6238. doi: 10.1038/s41598-017-06217-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Different treatment effects of HLJDT-M (a) and HLJDT (a) on the levels of APP metabolites, phospho-Tau and total Tau in 3XTg-AD mice. Immunoblot presenting the levels of FL-APP, CTFs (CTFα and CTFβ), pAPP and pCTFs (α and β). Quantification of immunoblots by densitometric analysis and is presented as the ratio of FL-APP, CTFs, pAPP, PHF-1 Tau, total Tau and pCTFs against β-actin in the SDS brain lysates of 3XTgAD mice treated with HLJDT-M or HLJDT or vehicle (c and d). The statistical significance are denoted as *p < 0.05, **p < 0.01, **p < 0.001 when compared with vehicle-treated 3XTg-AD mice. Data represent mean ± SEM. N = 8 mice per group.