Table 1.

Specifications of antibodies used in this study.

Antibody (clone)	Region specificity	Antigen	Source	Use and Dilution
Rabbit polyclonal to APP CT695 (CT695)	Human, mouse and rat FL-APP and CTFs	C-terminus 22 amino acid residues of β-APP peptide	Thermoscientifi, Waltham, MA, USA	Western blotting (WB) 1:1000;
Mouse monoclonal to human Aβ1-16 (6E10)	human Aβ (hAβ)	Amino acids residues 1–17 of hAβ peptide	Biolegend, Dedham, MA, USA	ELISA capture: 4 μg/ml
Biotinylated mouse monoclonal to human Aβ17-24 (4G8)	hAβ	Amino acids residues 17–24 of hAβ peptide	Biolegend, Dedham, MA, USA	IHC 1:500;
Biotinylated mouse monoclonal to human Aβ1-40 (5C3)	C-terminus of hAβ1-40; does not crossreact with hAβ1-42	C-terminal hAβ1-40 peptide	Nano tools, Teningen, Germany	ELISA detection: 0.5 μg/ml
Biotinylated mouse monoclonal to human Aβ1-42 (8G7)	C-terminus of hAβ1-42; does not crossreact with hAβ1-40	C-terminal hAβ1-42 peptide	Nano tools, Teningen, Germany	ELISA detection: 0.5 μg/ml
Rabbit polyclonal to phosphorylated APP (Thr668)	Human Phosphorylated APP at Thr668	Phosphopepitopes matching to residues neighboring Thr668 of human APP695	Cell signaling, Danvers, MA, USA	WB 1:1000
Rabbit polyclonal to human BACE1 (ab2077)	Human and mouse BACE1	Amino acids residues 485–501 of Human BACE	Abcam, Cambridge, MA, USA	WB 1:1000
PHF-1 monoclonal to phospho Tau	Human, mouse and rat phospho Tau	Epitopes matching to residues neighboring Ser396 and Ser404 phosphorylated sites	Prof. Peter Davies Albert Einstein College of Medicine, Manhasset, NY, USA	WB 1:1000
HT7 monoclonal to total Tau	Human specific	Human Tau between residue 159 and 163	Thermoscientific	WB, 1:1000
Mouse monoclonal to β-actin (C4)	β-actin	Bird gizzard actin	Santa Cruz, Dallas, TX, USA	WB: 1:1000