Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 24;7:6228. doi: 10.1038/s41598-017-05875-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

CDKL5 is localized at the centrosome. (a) The indicated cells were stained as in Fig. 1. (b) Exogenously expressed GFP-CDKL5 (green) localizes at the γ-tubulin positive (red) centrosome in HeLa cells. DAPI staining (blue) was used to visualize DNA. (c) HeLa cells were transfected with siCDKL5#1 or a control siRNA (siCtr.); centrosomes were purified 60 h post-transfection and the obtained fractions analyzed by WB with Abs against CDKL5 and γ-tubulin. Input corresponds to approximately 0.6% of the whole cell extract before fractionation. Fractions 1 and 6 are the bottom and top ones, respectively (n = 3 independent experiments). Input and fractions 1–6 are shown as different exposures of the same membrane; full-length blots are presented in Supplementary Figure S7a. (d) Neurospheres (upper) and primary hippocampal neurons (DIV4; lower) were stained with Abs against CDKL5 (polyclonal, green) and either γ-tubulin or pericentrin (both red) and DAPI (blue). The panels to the right show the magnified centrosome. Scale bar, 10 µm.