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. 2017 Jul 24;7:6311. doi: 10.1038/s41598-017-06581-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Activation and differentiation markers in muscle regeneration. Densitometric analysis of Pax7 (A), Myf5 (C), MyoD (D), Myogenin (F), pSTAT3 (G) and pSTAT1 (H) protein expression levels in gastrocnemius. Data are normalized to endogenous control (α-tubulin) and expressed as arbitrary units (A.U.). Representative cropped blots of two animals of each group are shown, control and AiA, respectively. Full length blots are presented in Supplementary Figures 1 and 2. Data represent individual values and medians with IQRs are also indicated (n = 6 rabbits per group). Representative TA cross sections with Pax7 (B) and MyoD (E) immunoreactive nuclei (black arrows) in control (left) and AiA (right) groups. Scale bar = 25 μm. Pax7 = paired box protein 7, Myf5 = myogenic factor 5, MyoD = myogenic differentiation 1, pSTAT = phosphorylated signal transducer and activator of transcription.