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. 2017 Jul 24;7:6221. doi: 10.1038/s41598-017-06539-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differential gene expression in HSCR-NLBs versus Control-NLBs. (a) The Heat map was generated using DataAssist v3.0 software (Life Technologies) and it represents the messenger RNA expression levels of 9 genes expressed in colon tissue from HSCR patients and controls. Genes were hierarchically clustered by Pearson correlation coefficient using average linkage. The color scale, representing ΔCt, is shown on the right side. Green indicates genes with relatively decreased expression in HSCR, whereas red indicates genes with relatively increased expression in HSCR compared with the controls. Ct, cycle threshold; Hirschsprung patiens (H); Control (C). (b) Table showing the variations in the gene expression levels in the NLBs from HSCR patients compared with Controls.