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. 2017 Jul 19;8:15902. doi: 10.1038/ncomms15902

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Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Cryo-SEM micrographs showing the morphology of (from left to right) soluble NSF, NSF micrococoons, released and aggregated NSF. Scale bars, 100 μm; The corresponding images of NSF solutions are shown as inserts (left); (b) FTIR spectra of the aggregation and release of NSF by snap-freezing in liquid nitrogen. Insert: schematic representation of the formation of spherical NSF micrococoons and NSF release from micrococoons; (c) histogram showing the efficiency of NSF release by snap-freezing, gentle centrifugation and continuous washing of the NSF micrococoons. The indicated error bars are the s.d. of the average of three different repeats, each one measure 12 times. (d) Histogram of NSF secondary structure elements calculated from the amide I band in the FTIR spectra (b) after release from the microncapsules.