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. 2017 Jul 19;8:15878. doi: 10.1038/ncomms15878

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Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Immunohistochemistry in hippocampal sections reveals a subset of neurons that express TRPV1, reelin and somatostatin in the stratum oriens; scale bar, 100 μm, SO: stratum oriens, SP: stratum pyramidale, SR: stratum radiatum). (b) Immunostains of the stratum oriens in hippocampal sections from PV-Tomato reporter mice for TRPV1 and mGluR7 (left) and from VIP-Tomato reporter mice with TRPV1 and DAPI (right); scale bar, 100 μm. (c) Quantitation of co-localization of immunofluorescence signal of different markers with TRPV1 neurons in the stratum oriens of hippocampal sections. VR.5′ sv quantitation indicates signal from the C-terminal TRPV1 antibody, compared to the N-terminal TRPV1 antibody (Fig. 1g). Number of sections analysed: TRPV1/VR.5′ sv n=5, TRPV1/somatostatin n=3, TRPV1/reelin n=3, TRPV1/VIP n=3, TRPV1/PV n=5, TRPV1/GAD65 n=3, TRPV1/mGluR7 n=3.