Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 19;8:15878. doi: 10.1038/ncomms15878

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Quadruple immunocytochemistry images of hippocampal neurons immunostained for TRPV1, vGluT1, syt1 lumenal antibody internalized by recycling synaptic vesicles during depolarization, and MAP2, in the indicated conditions; scale bar, 20 μm. (b) Higher magnification images of vGluT1 and MAP2 on TRPV1-expressing neurons in hippocampal cultures; scale bar, 5 μm. (c) Quantification of excitatory synapse number on TRPV1-positive hippocampal neurons normalized to surrounding cells in the indicated conditions. Black asterisks indicate significance between TRPV1-expressing and surrounding neurons in each condition. Blue asterisks indicate significance relative to control for either TRPV1-expressing cells or surrounding cells. (d) Quantitation of vGluT1, and syt1 uptake (e) intensity in presynaptic terminals contacting TRPV1-expressing neurons, normalized to surrounding neurons, in the indicated conditions. Images used for quantitation were: WT control n=24, WT 50 nM cap. n=12, WT 1 μM cap. n=9, WT 1 μM cap. + 1 μM SB n=12, WT 1 μM SB n=12, TRPV1 KO control n=9, TRPV1 KO 1 μM cap. n=9; from five different cultures. (f) Immunostains of TRPV1, vGAT, lumenal syt1 antibody internalized by recycling synaptic vesicles during depolarization, and MAP2 in the indicated conditions; scale bars, 20 μm. (g) Higher magnification images of vGAT and MAP2 immunostaining; scale bars, 5 μm. (h) Quantitation of inhibitory synapse number (number of vGAT-positive puncta) on TRPV1-expressing neurons normalized to surrounding cells. (i) Quantitation of vGAT intensity in presynaptic terminals contacting TRPV1-expressing neurons, normalized to surrounding neurons, in the indicated conditions. (j) Quantitation of syt1 uptake in depolarizing conditions in vGAT-positive terminals contacting TRPV1-expressing neurons, normalized to surrounding neurons, in the indicated conditions. Images used for quantitation were: WT control n=23, WT 50 nM cap. n=11, WT 1 μM cap. n=9, WT 1 μM cap.+ 1 μM SB n=12, WT 1 μM SB n=12, TRPV1 KO control n=9, TRPV1 KO 1 μM cap. n=9; from 5 cultures. Error=s.e.m., significance determined by one-way ANOVA with Tukey's post hoc test for multiple comparisons, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001; fluorescence intensity and synapse number for all conditions was normalized to the corresponding average value for surrounding neurons in WT cell cultures.