Table 4.
Primers used in the present study.
| Name | Sequence (5′-3′) | Target | Reference |
|---|---|---|---|
| tetM_F | ACAGAAAGCTTATTATATAAC | tet(M) | 32 |
| tetM_R | TGGCGTGTCTATGATGTTCAC | ||
| tetO_F | AACTTAGGCATTCTGGCTCAC | tet(O) | This study |
| tetO_R | CTATGGACAACCCGACAGAAG | ||
| tetS_F | TAGATACTCCTGGACACAT | tet(S) | This study |
| tetS_R | ATGAGAATGACCTCGTTAC | ||
| tetW_F | CGGATTGTGGCATTTGT | tet(W) | This study |
| tetW_R | GCATAGAGGGTGAAGGAG | ||
| tetT_F | AAGGTTTATTATATAAAAGTG | tet(T) | 34 |
| tetT_R | AGGTGTATCTATGATATTTAC | ||
| tetA_F | GTAATTCTGAGCACTGTCGC | tet(A) | 32 |
| tetA_R | CTGCCTGGACAACATTGCTT | ||
| tetB_F | AAAACTTATTATATTATAGTG | tet(B) | 34 |
| tetB_R | TGGAGTATCAATAATATTCAC | ||
| tetC_F | TCTAACAATGCGCTCATCGT | tet(C) | 32 |
| tetC_R | CGTTGAAGGCTCTCAAGGGC | ||
| tetD_F | ATTACACTGCTGGACGCGAT | tet(D) | 32 |
| tetD_R | CTGATCAGCAGACAGATTGC | ||
| tetE_F | GTGATGATGGCACTGGTCAT | tet(E) | 32 |
| tetE_R | CTCTGCTGTACATCGCTCTT | ||
| tetG_F | TTTCGGATTCTTACGGTC | tet(G) | 32 |
| tetG_R | TCCTGCGATAGAGCTTAGA | ||
| tetK_F | TTATGGTGGTTGTAGCTAGAAA | tet(K) | 33 |
| tetK_R | AAAGGGTTAGAAACTCTTGAAA | ||
| tetL_F | GTMGTTGCGCGCTATATTCC | tet(L) | 33 |
| tetL_R | GTGAAMGRWAGCCCACCTAA | ||
| tetW_U_SP1 | GGAGGTTGTTTCCGCTTTGCTG | Upstream region of tet(W) | This study |
| tetW_U_SP2 | GGTAAAGGAACCCACCGTCATT | ||
| tetW_U_SP3 | TCTGTTACACCACTCCCGCTTG | ||
| tetW_D_SP1 | CATCTGTGCCACTGGAAGGAAG | Downstream region of tet(W) | This study |
| tetW_D_SP2 | TCCGTCCTCGTTGTCCCTTTTT | ||
| tetW_D_SP3 | AAGGTCGTCTTTCCAGCGTCTA | ||
| tetO_U_SP1 | GCAAATCAATCCCCTCTTGGTCA | Upstream region of tet(O) | This study |
| tetO_U_SP2 | GTCTGTGCCTGTATGCCATCCTTT | ||
| tetO_U_SP3 | CCACTGAAAAGATGTCACTGCTGT | ||
| tetO_D1_SP1 | CGATACAGCCTGCTCTGGTGAT | Downstream region of the1457-bp tet(O) | This study |
| tetO_D1_SP2 | CTCCCTATGCTCCAAACAACGA | ||
| tetO_D1_SP3 | TATTGCTTGGGGCACTTACAGA | ||
| tetO_D2_SP1 | TTTCTGGGCTTCTGTCGGGTTGTC | Downstream region of the 1800-bp tet(O) | This study |
| tetO_D2_SP2 | AAATGCGGTTATGGAGGGGGTTCT | ||
| tetO_D2_SP3 | GCAGGGACAGAACTATTAGAGCCA | ||
| tetS_U_SP1 | GATAGCGGTACAACGAAAACGGTA | Upstream region of tet(S) | This study |
| tetS_U_SP2 | TTTGGAACGCCAGAGAGGTATT | ||
| tetS_U_SP3 | CTGGACATGGATTTTTGGCAG | ||
| tetS_D_SP1 | TGCCAAAATCCATGGTCCAGG | Downstream region of tet(S) | This study |
| tetS_D_SP2 | CGGTCTGAATAGTAATACCTGTGTGG | ||
| tetS_D_SP3 | CCGTTTTGGTTGTACCGCTATC |