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Sequences and annealing data for fluorescently labeled RNA oligonucleotides used for a double-reporter RNA unwinding experiment (nucleotide modified with Cy3 in red; nucleotide modified with BHQ in blue).
| Single-stranded overhang (nt) | Duplex length (Cy3, bp) | Annealing ΔG (Cy3, kcal/mol) | Helicase substrate |
|---|---|---|---|
| 20 | 24 | −24.7 |
|
