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. Author manuscript; available in PMC: 2017 Jul 25.
Published in final edited form as: Nat Protoc. 2014 Jun 19;9(7):1645–1661. doi: 10.1038/nprot.2014.112
Component Percentage
16% 12% 10% 8% 6%
Acrylamide, Bis-acrylamide 40% wt/vol (29:1) 24 ml 18 ml 15 ml 12 ml 9 ml
TBE (5X) 12 ml 12 ml 12 ml 12 ml 12 ml
Urea 30 g 30 g 30 g 30 g 30 g
H2O – 6 ml 9 ml 12 ml 15 ml
10% (wt/vol) ammonium persulfate 400 μl 400 μl 400 μl 400 μl 400 μl
TEMED 60 μl 60 μl 60 μl 60 μl 60 μl
Total Volume 60 ml 60 ml 60 ml 60 ml 60 ml