| Component and Concentration | Volume (μl) | Final Concentration |
|---|---|---|
| 1 μM Cy3-labeled RNA | 3.75 | 50 nM |
| 1 μM Loading RNA | 3.75 | 50 nM |
| 5 μM DNA competitor | 3.75 | 500 nM |
| 10 μM eIF4A (Helicase) | 7.5 | 1 μM |
| 20 mM ATP | 7.5 | 2 mM |
| 1x Reaction buffer | 48.75 | 1x |
|
| ||
| Total | 75 | |
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