| Component and Concentration | Volume (μl) | Final Concentration |
|---|---|---|
| 250 nM annealed substrate | 15 | 50 nM |
| 10 μM helicase | 7.5 | 1 μM |
| 1x Reaction buffer | 45 | 1x |
|
| ||
| Total | 67.5 | |
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