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. Author manuscript; available in PMC: 2017 Sep 6.
Published in final edited form as: Biochemistry. 2016 Aug 19;55(35):4885–4908. doi: 10.1021/acs.biochem.6b00293

Figure 10.

Figure 10

Clostridia tetani neurotoxin (TeNT) denaturation profile. His-tagged TeNT (through light chain catalytic domain) (at 0.2 μM concentration for 1 nm loading deflection) was attached onto Ni-NTA tips, and a denaturant pulse procedure was performed. The kinetic isotherms were generated in the absence (black circles) and presence (gray circles) of 1 μM GT1b. Control sensorgrams (no GroEL) exhibiting no contributions from GT1b dissociation were observed over the time course of the GroEL binding phase. A small amount of GroEL binding to the immobilized TeNT was detected under non-denaturing conditions (0 M urea).