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A) Kinetic stability isotherm of von Willebrand factor triple A domain generated with BLI denaturant pulse assay:time dependent left shift of load corrected GroEL binding response as a function of denaturant concentration. B) Comparison of the kinetically controlled denaturation profile generated under10 minute kinetic denaturant pulse (black circles) to equilibrium denaturation profile with 95% confidence interval (grey shaded region).
