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. 2017 Jul 21;8:15977. doi: 10.1038/ncomms15977

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Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Targeting of OSN axons and MC migration in the Nrp2 KOs. Parasagittal MOB sections at P0 were analysed by immunohistochemistry (upper panel). In the KOs, a coding sequence of the Nrp2 has been deleted and replaced with that of GFP. Thus, OSNs that are supposed to express Nrp2 can be detected with anti-GFP antibodies. Parasagittal sections at E18 were also analysed by in situ hybridization using the GFP and Nrp2 probes (lower panel). Dotted lines in the enlarged photos indicate the boundary of the GL and MCL. n=7, 10, 6 and 8 for the control, total KO, OSN-specific cKO and MC-specific cKO, respectively. (b) Targeting of OSN axons and MC migration in the Sema3F KOs. Parasagittal MOB sections at P0 were immunostained with anti-Nrp2 antibodies (upper panel). In the Sema3F total KO and OSN-specific Sema3F conditional KO (OSN cKO), Nrp2⁺ OSNs are defasciculated and mistargeted to the dorsal MOB. Dotted lines in the enlarged photos indicate the boundary of the GL and MCL. Parasagittal MOB sections at E18 were also analysed by in situ hybridization with the Nrp2 probe (lower panel). n=7, 9 and 6 for the control, total KO and OSN-specific cKO, respectively. Scale bar, 200 μm. D, dorsal; V, ventral; A, anterior; P, posterior.