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. 2017 Jul 21;8:15977. doi: 10.1038/ncomms15977

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Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) DNA constructs to detect the Nrp2⁺ MC axons (left). The BAC Tg mouse Nrp2-Cre was crossed with another Tg mouse Pcdh21-lacZ-STOP-tauYFP. Since Nrp2 was difficult to detect in MOB MCs, Tg mice, in which the Nrp2-expressing MCs were labelled with EYFP, were generated. The lacZ was inserted into the construct to detect the promoter activity of Pcdh21 by X-gal staining. Whole mount staining of the MOB is shown (right). Locations of coronal sections (AON, PIR and MeA) analysed in b are indicated. (b) Detection of Nrp2⁺ MC axons in the OC. Coronal sections of the OC at P14 were immunostained with anti-GFP antibodies to detect EYFP expressed in the Nrp2⁺ MCs (left). Nrp2⁺ ventral MC axons (green) project to the AON, PIR, MeA and CoA. Coronal OC sections at P0 were immunostained with anti-OCAM antibodies (right). OCAM⁺ dorsal MC axons (red) project to the AON and PIR, but not to the MeA. n=6. Scale bar, 100 μm. Nuclei are counterstained with DAPI in blue.