Figure 5.

Metabolite changes in JA and traumatic acid pathways and stomatal low CO₂ response of A. thaliana mutants. (A) Changes of metabolites in JA and traumatic acid pathways in B. napus guard cells under low CO₂ treatment. 13-HPOT, (9Z,11E,15Z)-(13S)-13-Hydroperoxyoctadeca-9,11,15-trienoic acid; 12-OPDA, (15Z)-12-Oxophyto-10,15-dienoic acid; (B) Stomatal movement of JA biosynthesis and signaling mutants after low CO₂ treatment. Data are mean ± standard error of three independent experiments (n = 3) with 60 stomata counted for each replicate (i.e., a total of 180 stomata). Two-way ANOVA and Tukey's test were used for stomatal movement analysis between different time points and different genotypes; (C) Representative images of stomatal aperture of (B).