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. 2017 Jul 25;8:1411. doi: 10.3389/fmicb.2017.01411

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Copyright © 2017 Liu, Chen, Chen, Tsai and Huang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Purification of recombinant TcdB and the composition of NP-rTcdBs. (A) Functional domains of TcdB and regions included for nanoparticle vaccine construction. (B) Coomassie blue staining of purified rTcdB. rTcdB were purified from E. coli by His-tag affinity chromatography. Lanes 1 and 2: Total E. coli lysates containing rTcdB and unbound proteins. Lanes 3 and 4: Purified rTcdB with. (C) Immunoblotting confirmation of rTcdB. M. Protein markers. rTcdB from SDS-PAGE was transferred to a membrane and detected by anti-His (lane 1) and anti-TcdB (lane 2). (D) Composition of NP-rTcdBs. The ionized chitosan and γ–PGA were able to form polyelectrolyte complexes via electrostatic interactions, resulting in a matrix structure with a spherical shape.