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(a) Representative photomicrograph of myocardial necrosis in SEN and INT areas. HE-stained sections under 400x magnification. Arrows indicate nuclear pyknosis, karyolysis, cytoplasmatic changes, and hypereosinophilia. Bars: 50 μm. Western blot protein expression analysis depicting proapoptotic caspase 3 (b) and BAX (c) and antiapoptotic Bcl-2 (d) and HIF1-α (e) performed 8 weeks after diabetes induction. #p < 0.01 and †p < 0.001 versus the CT group. Data expressed in mean ± SEM.
