Table 1.
Sequences of the primers used in this study. The primers containing mixed nucleotides were designed for detection of both A/PR8 and A/WSN-derived RNAs. Influenza virus mRNAs utilise primers that are 10–13 nt in length and the predicted sizes of the mRNA fragments reflect this heterogeneity
| Target RNA | Primer specificity | Primer sequence | Expected product Size/s (nt) |
|---|---|---|---|
| Seg 1-GFP | c/mRNA | GGACACGCTGAACTTGTGG | 142/152–5 |
| vRNA | AGATAAGAGGATAATGGAAATG | 242 | |
| DI 1/244 | c/mRNA | ATATGGTCCACKGTGGTTTTTG | 110/120–3 |
| vRNA | GGAGAAGACTGAGGGGATTC | 252 | |
| Segment 2 (PB1) | c/mRNA* | TCCATGGTGTATCCTGTTCC | 146/156–9 |
| vRNA* | TGATTTCGAATCTGGAAGGA | 134 | |
| Segment 3 (PA) | c/mRNA* | TGAGTGCATATTGCTGCAAAT | 146/156–9 |
| vRNA* | TTCTTATCGTTCAGGCTCTT | 213 | |
| Segment 6 (NA) | c/mRNA* | TCCAGTATGGTTTTGAYTTCCR | 160/170–3 |
| vRNA* | TGGACTAGTGSGAGCATSAT | 130 | |
| 5S rRNA* | TCCCAGGCGGTCTCCCATCC | 100 |
* indicates primers taken from [50]