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Linearity, PCR Efficiency and LOD of the pan-Alphavirus RT-PCR assay
| CHIKV | WEEV | EEEV | VEEV | BFV | |
|---|---|---|---|---|---|
| R2 | 0.994 | 0.998 | 0.995 | 0.993 | 0.991 |
| Slope | −3.39 | −3.81 | −3.77 | −3.55 | −3.67 |
| Intercept | 39.49 | 44.58 | 46.92 | 43.35 | 41.10 |
| Efficiency (%) | 97.24 | 82.99 | 84.18 | 91.35 | 87.12 |
| LOD (copies/run) | 26.6 | 59.5 | 42.6 | 111.2 | 87.5 |
