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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Cancer Immunol Res. 2017 May 4;5(6):439–445. doi: 10.1158/2326-6066.CIR-16-0364

Figure 2. Enhanced cytolytic function of TNBC TILs generated with anti-4-1BB.

Figure 2

TNBC TILs grown with IL2 alone or with IL2 + anti-4-1BB for 28 days from 5 independent lines were cocultured with target P815 (DDAO-SE labeled) cells pulsed with anti-CD3 (OKT3, diamonds). P815 cells not pulsed with OKT3 were used as a negative control (squares). Cells were stained for active caspase-3 by flow cytometry as a measure of tumor killing by TIL effectors. Gating was performed on DDAO-SE–positive target cells to determine the level of active caspase-3 detection. The graphs show the percentage of caspase-3 positive tumor targets at decreasing TIL: tumor ratios. Each experiment was independently performed per patient.