Figure 4.

Track analysis for anterograde and retrograde moving mitochondria in control white larvae, dhtt^−/−, and dhtt^−/− expressing a dhtt full-length rescue construct. Tracks represent three states of mitochondrial movement for an individual mitochondrion tracked over the entire track length, including movement in the primary direction, reverse direction, and stops. Anterograde or retrograde designations reflect the imprinted or primary direction of movement. (A) Loss of dHtt results in an increase in mitochondrial stops, indicating a defect in processivity. (B) Loss of dHtt results in an increase in mitochondrial run reversals, indicating a defect in motor coordination. N > 10 larvae per genotype and n > 12 mitochondria per larvae for all panels. Error bars represent SEM, *indicates p < 0.05.